Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC9B1 / NHEDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107087-100
Catalog Number: LS-G107087
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NHEDC1(SLC9B1) (NM_139173), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NHA1 | SLC9B1 | NHEDC1 | Sodium/hydrogen exchanger 9B1
- Origin Species
- Human
- SwissProt
- Q4ZJI4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHTTESKNEH LEDENFQTST TPQSLIDPNN TAHEETKTVL SDTEEIKPQT KKETYISCPL61RGVLNVIITN GVILFVIWCM TWSILGSEAL PGGNLFGLFI IFYSAIIGGK ILQLIRIPLV121PPLPPLLGML LAGFTIRNVP FINEHVHVPN TWSSILRSIA LTIILIRAGL GLDPQALRHL181KVVCFRLAVG PCLMEASAAA VFSHFIMKFP WQWAFLLGFV LGAVSPAVVV PYMMVLQENG241YGVEEGIPTL LMAASSMDDI LAITGFNTCL SIVFSSGGIL NNAIASIRNV CISLLAGIVL301GFFVRYFPSE DQKKLTLKRG FLVLTMCVSA VLGSQRIGLH GSGGLCTLVL SFIAGTKWSQ361EKMKVQKIIT TVWDIFQPLL FGLVGAEVSV SSLESNIVGI SVATLSLALC VRILTTYLLM421CFAGFSFKEK IFIALAWMPK ATVQAVLGPL ALETARVSAP HLEPYAKDVM TVAFLAILIT481APNGALLMGI LGPKMLTRHY DPSKIKLQLS TLEHHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
515 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.