Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC9A5 / NHE5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116535-100
Catalog Number: LS-G116535
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC9A5 (NM_004594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NHE5 | Sodium/hydrogen exchanger 5 | Na(+)/H(+) exchanger 5 | NHE-5 | SLC9A5
- Origin Species
- Human
- NCBI GenBank
- NM_004594
- SwissProt
- Q14940
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRAALSLLA LPLAGAAEEP TQKPESPGEP PPGLELFRWQ WHEVEAPYLV ALWILVASLA61KIVFHLSRKV TSLVPESCLL ILLGLVLGGI VLAVAKKAEY QLEPGTFFLF LLPPIVLDSG121YFMPSRLFFD NLGAILTYAV VGTLWNAFTT GAALWGLQQA GLVAPRVQAG LLDFLLFGSL181ISAVDPVAVL AVFEEVHVNE TLFIIVFGES LLNDAVTVVL YKVCNSFVEM GSANVQATDY241LKGVASLFVV SLGGAAVGLV FAFLLALTTR FTKRVRIIEP LLVFLLAYAA YLTAEMASLS301AILAVTMCGL GCKKYVEANI SHKSRTTVKY TMKTLASCAE TVIFMLLGIS AVDSSKWAWD361SGLVLGTLIF ILFFRALGVV LQTWVLNQFR LVPLDKIDQV VMSYGGLRGA VAFALVILLD421RTKVPAKDYF VATTIVVVFF TVIVQGLTIK PLVKWLKVKR SEHHKPTLNQ ELHEHTFDHI481LAAVEDVVGH HGYHYWRDRW EQFDKKYLSQ LLMRRSAYRI RDQIWDVYYR LNIRDAISFV541DQGGHVLSST GLTLPSMPSR NSVAETSVTN LLRESGSGAC LDLQVIDTVR SGRDREDAVM601HHLLCGGLYK PRRRYKASCS RHFISEDAQE RQDKEVFQQN MKRRLESFKS TKHNICFTKS661KPRPRKTGRR KKDGVANAEA TNGKHRGLGF QDTAAVILTV ESEEEEEESD SSETEKEDDE721GIIFVARATS EVLQEGKVSG SLEVCPSPRI IPPSPTCAEK ELPWKSGQGD LAVYVSSETT781KIVPVDMQTG WNQSISSLES LASPPCNQAP ILTCLPPHPR GTEEPQVPLH LPSDPRSSFA841FPPSLAKAGR SRSESSADLP QQQELQPLMG HKDHTHLSPG TATSHWCIQF NRGSRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
896 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.