Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC8A3 / NCX3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107923-100
Catalog Number: LS-G107923
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC8A3 (NM_058240), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Na+/Ca2+ exchanger 3 | NCX2 | NCX3 | Sodium-calcium exchanger 3 | Sodium/calcium exchanger 3 | SLC8A3
- Origin Species
- Human
- NCBI GenBank
- NM_033262
- SwissProt
- P57103
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAWLRLQPLT SAFLHFGLVT FVLFLNGLRA EAGGSGDVPS TGQNNESCSG SSDCKEGVIL61PIWYPENPSL GDKIARVIVY FVALIYMFLG VSIIADRFMA SIEVITSQER EVTIKKPNGE121TSTTTIRVWN ETVSNLTLMA LGSSAPEILL SLIEVCGHGF IAGDLGPSTI VGSAAFNMFI181IIGICVYVIP DGETRKIKHL RVFFITAAWS IFAYIWLYMI LAVFSPGVVQ VWEGLLTLFF241FPVCVLLAWV ADKRLLFYKY MHKKYRTDKH RGIIIETEGD HPKGIEMDGK MMNSHFLDGN301LVPLEGKEVD ESRREMIRIL KDLKQKHPEK DLDQLVEMAN YYALSHQQKS RAFYRIQATR361MMTGAGNILK KHAAEQAKKA SSMSEVHTDE PEDFISKVFF DPCSYQCLEN CGAVLLTVVR421KGGDMSKTMY VDYKTEDGSA NAGADYEFTE GTVVLKPGET QKEFSVGIID DDIFEEDEHF481FVRLSNVRIE EEQPEEGMPP AIFNSLPLPR AVLASPCVAT VTILDDDHAG IFTFECDTIH541VSESIGVMEV KVLRTSGARG TVIVPFRTVE GTAKGGGEDF EDTYGELEFK NDETVKTIRV601KIVDEEEYER QENFFIALGE PKWMERGISA LLLSPDRKLT MEEEEAKRIA EMGKPVLGEH661PKLEVIIEES YEFKTTVDKL IKKTNLALVV GTHSWRDQFM EAITVSAAGD EDEDESGEER721LPSCFDYVMH FLTVFWKVLF ACVPPTEYCH GWACFAVSIL IIGMLTAIIG DLASHFGCTI781GLKDSVTAVV FVAFGTSVPD TFASKAAALQ DVYADASIGN VTGSNAVNVF LGIGLAWSVA841AIYWALQGQE FHVSAGTLAF SVTLFTIFAF VCISVLLYRR RPHLGGELGG PRGCKLATTW901LFVSLWLLYI LFATLEAYCY IKGFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
924 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.