Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC7A3 / CAT-3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108626-100
Catalog Number: LS-G108626
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC7A3 (NM_032803), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATRC3 | CAT3 | SLC7A3 | CAT-3
- Origin Species
- Human
- NCBI GenBank
- NM_032803
- SwissProt
- Q8WY07
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPWQAFRRFG QKLVRRRTLE SGMAETRLAR CLSTLDLVAL GVGSTLGAGV YVLAGEVAKD61KAGPSIVICF LVAALSSVLA GLCYAEFGAR VPRSGSAYLY SYVTVGELWA FTTGWNLILS121YVIGTASVAR AWSSAFDNLI GNHISKTLQG SIALHVPHVL AEYPDFFALG LVLLLTGLLA181LGASESALVT RVFTGVNLLV LGFVMISGFV KGDVHNWKLT EEDYELAMAE LNDTYSLGPL241GSGGFVPFGF EGILRGAATC FYAFVGFDCI ATTGEEAQNP QRSIPMGIVI SLSVCFLAYF301AVSSALTLMM PYYQLQPESP LPEAFLYIGW APARYVVAVG SLCALSTSLL GSMFPMPRVI361YAMAEDGLLF RVLARIHTGT RTPIIATVVS GIIAAFMAFL FKLTDLVDLM SIGTLLAYSL421VSICVLILRY QPDQETKTGE EVELQEEAIT TESEKLTLWG LFFPLNSIPT PLSGQIVYVC481SSLLAVLLTA LCLVLAQWSV PLLSGDLVWT AVVVLLLLLI IGIIVVIWRQ PQSSTPLHFK541VPALPLLPLM SIFVNIYLMM QMTAGTWARF GVWMLIGFAI YFGYGIQHSL EEIKSNQPSR601KSRAKTVDLD PGTLYVHSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
619 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.