Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC7A10 / Asc-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111399-100
Catalog Number: LS-G111399
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC7A10 (NM_019849), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASC1 | ASC-1 | HASC-1 | SLC7A10
- Origin Species
- Human
- NCBI GenBank
- NM_019849
- SwissProt
- Q9NS82
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGHTQQPSG RGNPRPAPSP SPVPGTVPGA SERVALKKEI GLLSACTIII GNIIGSGIFI61SPKGVLEHSG SVGLALFVWV LGGGVTALGS LCYAELGVAI PKSGGDYAYV TEIFGGLAGF121LLLWSAVLIM YPTSLAVISM TFSNYVLQPV FPNCIPPTTA SRVLSMACLM LLTWVNSSSV181RWATRIQDMF TGGKLLALSL IIGVGLLQIF QGHFEELRPS NAFAFWMTPS VGHLALAFLQ241GSFAFSGWNF LNYVTEEMVD ARKNLPRAIF ISIPLVTFVY TFTNIAYFTA MSPQELLSSN301AVAVTFGEKL LGYFSWVMPV SVALSTFGGI NGYLFTYSRL CFSGAREGHL PSLLAMIHVR361HCTPIPALLV CCGATAVIML VGDTYTLINY VSFINYLCYG VTILGLLLLR WRRPALHRPI421KVNLLIPVAY LVFWAFLLVF SFISEPMVCG VGVIIILTGV PIFFLGVFWR SKPKCVHRLT481ESMTHWGQEL CFVVYPQDAP EEEENGPCPP SLLPATDKPS KPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
523 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.