Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC6A15 / SBAT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103959-100
Catalog Number: LS-G103959
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC6A15 (NM_182767), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Orphan transporter v7-3 | Transporter v7-3 | NTT73 | SBAT1 | SLC6A15 | B0AT2 | Hv7-3 | V7-3
- Origin Species
- Human
- NCBI GenBank
- NM_018057
- SwissProt
- Q9H2J7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPKNSKVVKR ELDDDVTESV KDLLSNEDAA DDAFKTSELI VDGQEEKDTD VEEGSEVEDE61RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL LPYLILLMVI GIPLFFLELS121VGQRIRRGSI GVWNYISPKL GGIGFASCVV CYFVALYYNV IIGWSLFYFS QSFQQPLPWD181QCPLVKNASH TFVEPECEQS SATTYYWYRE ALNISSSISE SGGLNWKMTI CLLAAWVMVC241LAMIKGIQSS GKIIYFSSLF PYVVLICFLI RAFLLNGSID GIRHMFTPKL EIMLEPKVWR301EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL VVFAVLGFKA361NVINEKCITQ NSETIMKFLK MGNISQDIIP HHINLSTVTA EDYHLVYDII QKVKEEEFPA421LHLNSCKIEE ELNKAVQGTG LAFIAFTEAM THFPASPFWS VMFFLMLVNL GLGSMFGTIE481GIVTPIVDTF KVRKEILTVI CCLLAFCIGL IFVQRSGNYF VTMFDDYSAT LPLLIVVILE541NIAVCFVYGI DKFMEDLKDM LGFAPSRYYY YMWKYISPLM LLSLLIASVV NMGLSPPGYN601AWIEDKASEE FLSYPTWGLV VCVSLVVFAI LPVPVVFIVR RFNLIDDSSG NLASVTYKRG661RVLKEPVNLE GDDTSLIHGK IPSEMPSPNF GKNIYRKQSG SPTLDTAPNG RYGIGYLMAD721IMPDMPESDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
730 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.