Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC6A13 / GAT-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G131002-100
Catalog Number: LS-G131002
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC6A13 (NM_001190997), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GAT-2 | GABA transport protein | GAT2 | SLC6A13
- Origin Species
- Human
- NCBI GenBank
- NM_016615
- SwissProt
- Q9NSD5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSRVSGTTS NGETKPVYPV MEKKEEDGTL ERGHWNNKME FVLSVAGEII GLGNVWRFPY61LCYKNGGEHC MEFQKTNGSL NGTSENATSP VIEFWERRVL KISDGIQHLG ALRWELALCL121LLAWVICYFC IWKGVKSTGK VVYFTATFPY LMLVVLLIRG VTLPGAAQGI QFYLYPNLTR181LWDPQVWMDA GTQIFFSFAI CLGCLTALGS YNKYHNNCYR DCIALCFLNS GTSFVAGFAI241FSILGFMSQE QGVPISEVAE SGPGLAFIAY PRAVVMLPFS PLWACCFFFM VVLLGLDSQF301VCVESLVTAL VDMYPHVFRK KNRREVLILG VSVVSFLVGL IMLTEGGMYV FQLFDYYAAS361GMCLLFVAIF ESLCVAWVYG AKRFYDNIED MIGYRPWPLI KYCWLFLTPA VCTATFLFSL421IKYTPLTYNK KYTYPWWGDA LGWLLALSSM VCIPAWSLYR LGTLKGPFRE RIRQLMCPAE481DLPQRNPAGP SAPATPRTSL LRLTELESHCSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
510 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.