Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC6A11 / GAT-3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114085-100
Catalog Number: LS-G114085
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC6A11(SLC6A11) (NM_014229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GAT-3 | GAT3 | GABT3 | GAT4 | SLC6A11
- Origin Species
- Human
- NCBI GenBank
- NM_014229
- SwissProt
- P48066
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAEKALPLG NGKAAEEARE SEAPGGGCSS GGAAPARHPR VKRDKAVHER GHWNNKVEFV61LSVAGEIIGL GNVWRFPYLC YKNGGGAFLI PYVVFFICCG IPVFFLETAL GQFTSEGGIT121CWRKVCPLFE GIGYATQVIE AHLNVYYIII LAWAIFYLSN CFTTELPWAT CGHEWNTENC181VEFQKLNVSN YSHVSLQNAT SPVMEFWEHR VLAISDGIEH IGNLRWELAL CLLAAWTICY241FCIWKGTKST GKVVYVTATF PYIMLLILLI RGVTLPGASE GIKFYLYPDL SRLSDPQVWV301DAGTQIFFSY AICLGCLTAL GSYNNYNNNC YRDCIMLCCL NSGTSFVAGF AIFSVLGFMA361YEQGVPIAEV AESGPGLAFI AYPKAVTMMP LSPLWATLFF MMLIFLGLDS QFVCVESLVT421AVVDMYPKVF RRGYRRELLI LALSVISYFL GLVMLTEGGM YIFQLFDSYA ASGMCLLFVA481IFECICIGWV YGSNRFYDNI EDMIGYRPPS LIKWCWMIMT PGICAGIFIF FLIKYKPLKY541NNIYTYPAWG YGIGWLMALS SMLCIPLWIC ITVWKTEGTL PEKLQKLTTP STDLKMRGKL601GVSPRMVTVN DCDAKLKSDG TIAAITEKET HFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
632 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.