Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC5A8 / AIT Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106523-100
Catalog Number: LS-G106523
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC5A8 (NM_145913), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIT | Apical iodide transporter | SLC5A8 | SMCT | SMCT1
- Origin Species
- Human
- NCBI GenBank
- NM_145913
- SwissProt
- Q8N695
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTPRGIGTF VVWDYVVFAG MLVISAAIGI YYAFAGGGQQ TSKDFLMGGR RMTAVPVALS61LTASFMSAVT VLGTPSEVYR FGAIFSIFAF TYFFVVVISA EVFLPVFYKL GITSTYEYLE121LRFNKCVRLC GTVLFIVQTI LYTGIVIYAP ALALNQVTGF DLWGAVVATG VVCTFYCTLG181GLKAVIWTDV FQVGIMVAGF ASVIIQAVVM QGGISTILND AYDGGRLNFW NFNPNPLQRH241TFWTIIIGGT FTWTSIYGVN QSQVQRYISC KSRFQAKLSL YINLVGLWAI LTCSVFCGLA301LYSRYHDCDP WTAKKVSAPD QLMPYLVLDI LQDYPGLPGL FVACAYSGTL STVSSSINAL361AAVTVEDLIK PYFRSLSERS LSWISQGMSV VYGALCIGMA ALASLMGALL QAALSVFGMV421GGPLMGLFAL GILVPFANSI GALVGLMAGF AISLWVGIGA QIYPPLPERT LPLHLDIQGC481NSTYNETNLM TTTEMPFTTS VFQIYNVQRT PLMDNWYSLS YLYFSTVGTL VTLLVGILVS541LSTGGRKQNL DPRYILTKED FLSNFDIFKK KKHVLSYKSH PVEDGGTDNP AFNHIELNSD601QSGKSNGTRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
610 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.