Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC5A4 / SGLT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114083-100
Catalog Number: LS-G114083
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC5A4 (NM_014227), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ90G24.4 | Na(+)/glucose cotransporter 3 | SAAT11 | Sodium transporter | Sodium/glucose cotransporter 3 | SLC5A4 | SAAT1 | SGLT3
- Origin Species
- Human
- NCBI GenBank
- NM_014227
- SwissProt
- Q9NY91
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTVSPSTI AETPEPPPLS DHIRNAADIS VIVIYFLVVM AVGLWAMLKT NRGTIGGFFL61AGRDMAWWPM GASLFASNIG SNHYVGLAGT GAASGVATVT FEWTSSVMLL ILGWIFVPIY121IKSGVMTMPE YLKKRFGGER LQVYLSILSL FICVVLLISA DIFAGAIFIK LALGLDLYLA181IFILLAMTAV YTTTGGLASV IYTDTLQTII MLIGSFILMG FAFNEVGGYE SFTEKYVNAT241PSVVEGDNLT ISASCYTPRA DSFHIFRDAV TGDIPWPGII FGMPITALWY WCTNQVIVQR301CLCGKDMSHV KAACIMCAYL KLLPMFLMVM PGMISRILYT DMVACVVPSE CVKHCGVDVG361CTNYAYPTMV LELMPQGLRG LMLSVMLASL MSSLTSIFNS ASTLFTIDLY TKMRKQASEK421ELLIAGRIFV LLLTVVSIVW VPLVQVSQNG QLIHYTESIS SYLGPPIAAV FVLAIFCKRV481NEQGAFWGLM VGLAMGLIRM ITEFAYGTGS CLAPSNCPKI ICGVHYLYFS IVLFFGSMLV541TLGISLLTKP IPDVHLYRLC WVLRNSTEER IDIDAEEKSQ EETDDGVEED YPEKSRGCLK601KAYDLFCGLQ KGPKLTKEEE EALSKKLTDT SERPSWRTIV NINAILLLAV VVFIHGYYATRTRPLEQKLISEEDLAANDILDYKDDDDKV
659 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.