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SLC5A3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SLC5A3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SLC5A3 antibodies →
$725.00each
Catalog Number: LS-G114977-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC5A3 (NM_006933), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Na+/myo-inositol cotransporter | SMIT1 | SLC5A3 | SMIT | BCW2 protein
Origin Species
Human
NCBI GenBank
NM_006933
SwissProt
P53794

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRAVLDTADI AIVALYFILV MCIGFFAMWK CNRSTVSGYF LAGRSMTWVA IGASLFVSNI
61GSEHFIGLAG SGAASGFAVG AWEFNALLLL QLLGWVFIPI YIRSGVYTMP EYLSKRFGGH
121RIQVYFAALS LILYIFTKLS VDLYSGALFI QESLGWNLYV SVILLIGMTA LLTVTGGLVA
181VIYTDTLQAL LMIIGALTLM IISIMEIGGF EEVKRRYMLA SPDVTSILLT YNLSNTNSCN
241VSPKKEALKM LRNPTDEDVP WPGFILGQTP ASVWYWCADQ VIVQRVLAAK NIAHAKGSTL
301MAGFLKLLPM FIIVVPGMIS RILFTDDIAC INPEHCMLVC GSRAGCSNIA YPRLVMKLVP
361VGLRGLMMAV MIAALMSDLD SIFNSASTIF TLDVYKLIRK SASSRELMIV GRIFVAFMVV
421ISIAWVPIIV EMQGGQMYLY IQEVADYLTP PVAALFLLAI FWKRCNEQGA FYGGMAGFVL
481GAVRLILAFA YRAPECDQPD NRPGFIKDIH YMYVATGLFW VTGLITVIVS LLTPPPTKEQ
541IRTTTFWSKK NLVVKENCSP KEEPYKMQEK SILRCSENNE TINHIIPNGK SEDSIKGLQP
601EDVNLLVTCR EEGNPVASLG HSEAETPVDA YSNGQAALMG EKERKKETDD GGRYWKFIDW
661FCGFKSKSLS KRSLRDLMEE EAVCLQMLEE TRQVKVILNI GLFAVCSLGI FMFVYFSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

718 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.