Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC5A11 / SMIT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108080-100
Catalog Number: LS-G108080
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC5A11 (NM_052944), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Homolog of rabbit KST1 | KST1 | SLC5A11 | SLGTX | SMIT2 | RKST1 | SGLT6
- Origin Species
- Human
- NCBI GenBank
- NM_052944
- SwissProt
- Q8WWX8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESGTSSPQP PQLDPLDAFP QKGLEPGDIA VLVLYFLFVL AVGLWSTVKT KRDTVKGYFL61AGGDMVWWPV GASLFASNVG SGHFIGLAGS GAATGISVSA YELNGLFSVL MLAWIFLPIY121IAGQVTTMPE YLRKRFGGIR IPIILAVLYL FIYIFTKISV DMYAGAIFIQ QSLHLDLYLA181IVGLLAITAV YTVAGGLAAV IYTDALQTLI MLIGALTLMG YSFAAVGGME GLKEKYFLAL241ASNRSENSSC GLPREDAFHI FRDPLTSDLP WPGVLFGMSI PSLWYWCTDQ VIVQRTLAAK301NLSHAKGGAL MAAYLKVLPL FIMVFPGMVS RILFPDQVAC ADPEICQKIC SNPSGCSDIA361YPKLVLELLP TGLRGLMMAV MVAALMSSLT SIFNSASTIF TMDLWNHLRP RASEKELMIV421GRVFVLLLVL VSILWIPVVQ ASQGGQLFIY IRSISSYLQP PVAVVFIMGC FWKRTNEKGA481FWGLISGLLL GLVRLVLDFI YVQPRCDQPD ERPVLVKSIH YLYFSMILST VTLITVSTVS541WFTEPPSKEM VSHLTWFTRH DPVVQKEQAP PAAPLSLTLS QNGMPEASSS SSVQFEMVQE601NTSKTHSCDM TPKQSKVVKA ILWLCGIQEK GKEELPARAE AIIVSLEENP LVKTLLDVNL661IFCVSCAIFI WGYFATRTRPLEQKLISEEDLAANDILDYKDDDDKV
675 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.