Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC4A9 / AE4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109193-100
Catalog Number: LS-G109193
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC4A9 (NM_031467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AE4 | Anion exchange protein 4 | AE 4 | Anion exchanger 4 | SLC4A9 | SBC5
- Origin Species
- Human
- NCBI GenBank
- AB032762
- SwissProt
- Q96Q91
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEMKLPGQEG FEASSAPRNI PSGELDSNPD PGTGPSPDGP SDTESKELGV PKDPLLFIQL61NELLGWPQAL EWRETGRWVL FEEKLEVAAG RWSAPHVPTL ALPSLQKLRS LLAEGLVLLD121CPAQSLLELV EQVTRVESLS PELRGQLQAL LLQRPQHYNQ TTGTRPCWGS THPRKASDNE181EAPLREQCQN PLRQKLPPGA EAGTVLAGEL GFLAQPLGAF VRLRNPVVLG SLTEVSLPSR241FFCLLLGPCM LGKGYHEMGR AAAVLLSDPQ FQWSVRRASN LHDLLAALDA FLEEVTVLPP301GRWDPTARIP PPKCLPSQHK RLPSQQREIR GPAVPRLTSA EDRHRHGPHA HSPELQRTGR361LFGGLIQDVR RKVPWYPSDF LDALHLQCFS AVLYIYLATV TNAITFGGLL GDATDGAQGV421LESFLGTAVA GAAFCLMAGQ PLTILSSTGP VLVFERLLFS FSRDYSLDYL PFRLWVGIWV481ATFCLVLVAT EASVLVRYFT RFTEEGFCAL ISLIFIYDAV GKMLNLTHTY PIQKPGSSAY541GCLCQYPGPG GNESQWIRTR PKDRDDIVSM DLGLINASLL PPPECTRQGG HPRGPGCHTV601PDIAFFSLLL FLTSFFFAMA LKCVKTSRFF PSVVRKGLSD FSSVLAILLG CGLDAFLGLA661TPKLMVPREF KPTLPGRGWL VSPFGANPWW WSVAAALPAL LLSILIFMDQ QITAVILNRM721EYRLQKGAGF HLDLFCVAVL MLLTSALGLP WYVSATVISL AHMDSLRRES RACAPGERPN781FLGIREQRLT GLVVFILTGA SIFLAPVLKF IPMPVLYGIF LYMGVAALSS IQFTNRVKLL841LMPAKHQPDL LLLRHVPLTR VHLFTAIQLA CLGLLWIIKS TPAAIIFPLM LLGLVGVRKA901LERVFSPQEL LWLDELMPEE ERSIPEKGLE PEHSFSGSDS EDSELMYQPK APEINISVNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
959 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.