Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC4A1AP / Kanadaptin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111933-100
Catalog Number: LS-G111933
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kanadaptin(SLC4A1AP) (NM_018158), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HLC-3 | Kanadaptin | Lung cancer oncogene 3 | HLC3 | SLC4A1AP
- Origin Species
- Human
- NCBI GenBank
- NM_018158
- SwissProt
- Q9BWU0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLAPLRNAPG REGATSPSPP TDATGSLGEW DVDRNVKTEG WVSKERISKL HRLRMADILS61QSETLASQDL SGDFKKPALP VSPAARSKAP ASSSSNPEEV QKEGPTALQD SNSGEPDIPP121PQPDCGDFRS LQEEQSRPTT AVSSPGGPAR APPYQEPPWG GPATAPYSLE TLKGGTILGT181RSLKGTSYCL FGRLSGCDVC LEHPSVSRYH AVLQHRASGP DGECDSNGPG FYLYDLGSTH241GTFLNKTRIP PRTYCRVHVG HVVRFGGSTR LFILQGPEED REAESELTVT QLKELRKQQQ301ILLEKKMLGE DSDEEEEMDT SERKINAGSQ DDEMGCTWGM GEDAVEDDAE ENPIVLEFRQ361EREAFYIKDP KKALQGFFDR EGEELEYEFD EQGHSTWLCR VRLPVDDSTG KQLVAEAIHS421GKKKEAMIQC SLEACRILDT LGLLRQEAVS RKRKAKNWED EDFYDSDDDT FLDRTGLIEK481KRLNRMKKAG KIDEKPETFE SLVAKLNDAE RELSEISERL KASSQVLSES PSQDSLDAFM541SEMKSGSTLD GVSRKKLHLR TFELRKEQQR LKGLIKIVKP AEIPELKKTE TQTTGAENKA601KKLTLPLFGA MKGGSKFKLK TGTVGKLPPK RPELPPTLMR MKDEPEVEEE EEEEEEEEKE661KEEHEKKKLE DGSLSRPQPE IEPEAAVQEM RPPTDLTHFK ETQTHENMSQ LSEEEQNKDY721QDCSKTTSLC AGPSASKNEY EKSRGELKKK KTPGPGKLPP TLSSKYPEDD PDYCVWVPPE781GQSGDGRTHL NDKYGYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
796 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.