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SLC47A2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SLC47A2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SLC47A2 antibodies →
$504.00each
Catalog Number: LS-G105934-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC47A2 (NM_152908), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MATE2 | MATE2-K | H+/organic cation antiporter | HMATE-2 | SLC47A2 | MATE-2 | MATE2-B | MATE2K
Origin Species
Human
SwissProt
Q86VL8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSLQDTVAL DHGGCCPALS RLVPRGFGTE MWTLFALSGP LFLFQVLTFM IYIVSTVFCG
61HLGKVELASV TLAVAFVNVC GVSVGVGLSS ACDTLMSQSF GSPNKKHVGV ILQRGALVLL
121LCCLPCWALF LNTQHILLLF RQDPDVSRLT QDYVMIFIPG LPVIFLYNLL AKYLQNQGWL
181KGQEEESPFQ TPGLSILHPS HSHLSRASFH LFQKITWPQV LSGVVGNCVN GVANYALVSV
241LNLGVRGSAY ANIISQFAQT VFLLLYIVLK KLHLETWAGW SSQCLQDWGP FFSLAVPSML
301MICVEWWAYE IGSFLMGLLS VVDLSAQAVI YEVATVTYMI PLGLSIGVCV RVGMALGAAD
361TVQAKRSAVS GVLSIVGISL VLGTLISILK NQLGHIFTND EDVIALVSQV LPVYSVFHVF
421EAICCVYGGV LRGTGKQAFG AAVNAITYYI IGLPLGILLT FVVRMRIMGL WLGMLVCVFL
481ATAAFVAYTA RLDWKLAAEE AKKHSGRQQQ QRAESTATRP GPEKAVLSSV ATGSSPGITL
541TTYSRSECHV DFFRTPEEAH ALSAPTSRLS VKQLVIRRGA ALGAASATLM VGLTVRILAT
601RH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.