Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC44A2 / CTL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111161-100
Catalog Number: LS-G111161
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC44A2 (NM_020428), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SLC44A2 | CTL2 | PP1292
- Origin Species
- Human
- NCBI GenBank
- NM_020428
- SwissProt
- Q8IWA5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDERPHYYG KHGTPQKYDP TFKGPIYNRG CTDIICCVFL LLAIVGYVAV GIIAWTHGDP61RKVIYPTDSR GEFCGQKGTK NENKPYLFYF NIVKCASPLV LLEFQCPTPQ ICVEKCPDRY121LTYLNARSSR DFEYYKQFCV PGFKNNKGVA EVLRDGDCPA VLIPSKPLAR RCFPAIHAYK181GVLMVGNETT YEDGHGSRKN ITDLVEGAKK ANGVLEARQL AMRIFEDYTV SWYWIIIGLV241IAMAMSLLFI ILLRFLAGIM VWVMIIMVIL VLGYGIFHCY MEYSRLRGEA GSDVSLVDLG301FQTDFRVYLH LRQTWLAFMI ILSILEVIII LLLIFLRKRI LIAIALIKEA SRAVGYVMCS361LLYPLVTFFL LCLCIAYWAS TAVFLSTSNE AVYKIFDDSP CPFTAKTCNP ETFPSSNESR421QCPNARCQFA FYGGESGYHR ALLGLQIFNA FMFFWLANFV LALGQVTLAG AFASYYWALR481KPDDLPAFPL FSAFGRALRY HTGSLAFGAL ILAIVQIIRV ILEYLDQRLK AAENKFAKCL541MTCLKCCFWC LEKFIKFLNR NAYIMIAIYG TNFCTSARNA FFLLMRNIIR VAVLDKVTDF601LFLLGKLLIV GSVGILAFFF FTHRIRIVQD TAPPLNYYWV PILTVIVGSY LIAHGFFSVY661GMCVDTLFLC FLEDLERNDG SAERPYFMSS TLKKPLNKTN KKAAESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
706 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.