Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC39A8 / ZIP8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101798-100
Catalog Number: LS-G101798
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BIGM103(SLC39A8) (NM_022154), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LZT-Hs6 | SLC39A8 | ZIP-8 | PP3105 | BIGM103 | ZIP8 | Zrt- and Irt-like protein 8 | Up-regulated by BCG-CWS | Zinc transporter ZIP8
- Origin Species
- Human
- NCBI GenBank
- NM_022154
- SwissProt
- Q9C0K1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPGRAVAGL LLLAAAGLGG VAEGPGLAFS EDVLSVFGAN LSLSAAQLQH LLEQMGAASR61VGVPEPGQLH FNQCLTAEEI FSLHGFSNAT QITSSKFSVI CPAVLQQLNF HPCEDRPKHK121TRPSHSEVWG YGFLSVTIIN LASLLGLILT PLIKKSYFPK ILTFFVGLAI GTLFSNAIFQ181LIPEAFGFDP KVDSYVEKAV AVFGGFYLLF FFERMLKMLL KTYGQNGHTH FGNDNFGPQE241KTHQPKALPA INGVTCYANP AVTEANGHIH FDNVSVVSLQ DGKKEPSSCT CLKGPKLSEI301GTIAWMITLC DALHNFIDGL AIGASCTLSL LQGLSTSIAI LCEEFPHELG DFVILLNAGM361STRQALLFNF LSACSCYVGL AFGILVGNNF APNIIFALAG GMFLYISLAD MFPEMNDMLR421EKVTGRKTDF TFFMIQNAGM LTGFTAILLI TLYAGEIELETRTRPLEQKLISEEDLAANDILDYKDDDDKV
460 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.