Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC39A5 / ZIP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105234-100
Catalog Number: LS-G105234
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC39A5 (NM_173596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LZT-Hs7 | SLC39A5 | ZIP5 | Zrt- and Irt-like protein 5 | ZIP-5 | Zinc transporter ZIP5
- Origin Species
- Human
- NCBI GenBank
- H37888
- SwissProt
- Q6ZMH5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSPVSHLLA GFCVWVVLGW VGGSVPNLGP AEQEQNHYLA QLFGLYGENG TLTAGGLARL61LHSLGLGRVQ GLRLGQHGPL TGRAASPAAD NSTHRPQNPE LSVDVWAGMP LGPSGWGDLE121ESKAPHLPRG PAPSGLDLLH RLLLLDHSLA DHLNEDCLNG SQLLVNFGLS PAAPLTPRQF181ALLCPALLYQ IDSRVCIGAP APAPPGDLLS ALLQSALAVL LLSLPSPLSL LLLRLLGPRL241LRPLLGFLGA LAVGTLCGDA LLHLLPHAQE GRHAGPGGLP EKDLGPGLSV LGGLFLLFVL301ENMLGLLRHR GLRPRCCRRK RRNLETRNLD PENGSGMALQ PLQAAPEPGA QGQREKNSQH361PPALAPPGHQ GHSHGHQGGT DITWMVLLGD GLHNLTDGLA IGAAFSDGFS SGLSTTLAVF421CHELPHELGD FAMLLQSGLS FRRLLLLSLV SGALGLGGAV LGVGLSLGPV PLTPWVFGVT481AGVFLYVALV DMLPALLRPP EPLPTPHVLL QGLGLLLGGG LMLAITLLEE RLLPVTTEGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
539 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.