Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC38A3 / SNAT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115049-100
Catalog Number: LS-G115049
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC38A3 (NM_006841), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SLC38A3 | G17 | SN1 | SNAT3
- Origin Species
- Human
- NCBI GenBank
- NM_006841
- SwissProt
- Q99624
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAPLQTEMV ELVPNGKHSE GLLPVITPMA GNQRVEDPAR SCMEGKSFLQ KSPSKEPHFT61DFEGKTSFGM SVFNLSNAIM GSGILGLAYA MANTGIILFL FLLTAVALLS SYSIHLLLKS121SGVVGIRAYE QLGYRAFGTP GKLAAALAIT LQNIGAMSSY LYIIKSELPL VIQTFLNLEE181KTSDWYMNGN YLVILVSVTI ILPLALMRQL GYLGYSSGFS LSCMVFFLIA VIYKKFHVPC241PLPPNFNNTT GNFSHVEIVK EKVQLQVEPE ASAFCTPSYF TLNSQTAYTI PIMAFAFVCH301PEVLPIYTEL KDPSKKKMQH ISNLSIAVMY IMYFLAALFG YLTFYNGVES ELLHTYSKVD361PFDVLILCVR VAVLTAVTLT VPIVLFPVRR AIQQMLFPNQ EFSWLRHVLI AVGLLTCINL421LVIFAPNILG IFGVIGATSA PFLIFIFPAI FYFRIMPTEK EPARSTPKIL ALCFAMLGFL481LMTMSLSFII IDWASGTSRH GGNHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
504 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.