Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC35D3 / FRCL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121882-100
Catalog Number: LS-G121882
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC35D3 (NM_001008783), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Frc | fringe-like 1 | FRCL1 | SLC35D3 | BA55K22.3
- Origin Species
- Human
- SwissProt
- Q5M8T2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRQLCRGRVL GISVAIAHGV FSGSLNILLK FLISRYQFSF LTLVQCLTSS TAALSLELLR61RLGLIAVPPF GLSLARSFAG VAVLSTLQSS LTLWSLRGLS LPMYVVFKRC LPLVTMLIGV121LVLKNGAPSP GVLAAVLITT CGAALAGAGD LTGDPIGYVT GVLAVLVHAA YLVLIQKASA181DTEHGPLTAQ YVIAVSATPL LVICSFASTD SIHAWTFPGW KDPAMVCIFV ACILIGCAMN241FTTLHCTYIN SAVTTSFVGV VKSIATITVG MVAFSDVEPT SLFIAGVVVN TLGSIIYCVA301KFMETRKQSN YEDLEAQPRG EEAQLSGDQL PFVMEELPGE GGNGRSEGGE AAGGPAQESR361QEVRGSPRGV PLVAGSSEEG SRRSLKDAYL EVWRLVRGTR YMKKDYLIEN EELPSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
416 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.