Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC35B1 / UGTREL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100654-100
Catalog Number: LS-G100654
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC35B1 (NM_005827), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HUGT2 | UGTREL1 | SLC35B1 | HUGTrel1
- Origin Species
- Human
- NCBI GenBank
- NM_005827
- SwissProt
- P78383
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSSLVPD RLRLPLCFLG VFVCYFYYGI LQEKITRGKY GEGAKQETFT FALTLVFIQC61VINAVFAKIL IQFFDTARVD HTRSWLYAAC SISYLGAMVS SNSALQFVNY PTQVLGKSCK121PIPVMLLGVT LLKKKYPLAK YLCVLLIVAG VALFMYKPKK VVGIEEHTVG YGELLLLLSL181TLDGLTGVSQ DHMRAHYQTG SNHMMLNINL WSTLLLGMGI LFTGELWEFL SFAERYPAII241YNILLFGLTS ALGQSFIFMT VVYFGPLTCS IITTTRKFFT ILASVILFAN PISPMQWVGT301VLVFLGLGLD AKFGKGAKKT SHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
322 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.