
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC30A3 / ZNT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100054-100
Catalog Number: LS-G100054
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC30A3 (NM_003459), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SLC30A3 | ZNT3 | Zinc transporter 3 | ZnT-3 | Zinc transporter ZnT-3
- Origin Species
- Human
- NCBI GenBank
- NM_003459
- SwissProt
- Q99726
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPSPAAGGL ETTRLVSPRD RGGAGGSLRL KSLFTEPSEP LPEESKPVEM PFHHCHRDPL61PPPGLTPERL HARRQLYAAC AVCFVFMAGE VVGGYLAHSL AIMTDAAHLL ADVGSMMGSL121FSLWLSTRPA TRTMTFGWHR SETLGALASV VSLWMVTGIL LYLAFVRLLH SDYHIEGGAM181LLTASIAVCA NLLMAFVLHQ AGPPHSHGSR GAEYAPLEEG PEEPLPLGNT SVRAAFVHVL241GDLLQSFGVL AASILIYFKP QYKAADPIST FLFSICALGS TAPTLRDVLR ILMEGTPRNV301GFEPVRDTLL SVPGVRATHE LHLWALTLTY HVASAHLAID STADPEAVLA EASSRLYSRF361GFSSCTLQVE QYQPEMAQCL RCQEPPQATRTRPLEQKLISEEDLAANDILDYKDDDDKV
388 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
