Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC2A7 / GLUT7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102814-100
Catalog Number: LS-G102814
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC2A7 (NM_207420), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Glucose transporter type 7 | GLUT-7 | GLUT7 | SLC2A7
- Origin Species
- Human
- SwissProt
- Q6PXP3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MENKEAGTPP PIPSREGRLQ PTLLLATLSA AFGSAFQYGY NLSVVNTPHK VFKSFYNETY61FERHATFMDG KLMLLLWSCT VSMFPLGGLL GSLLVGLLVD SCGRKGTLLI NNIFAIIPAI121LMGVSKVAKA FELIVFSRVV LGVCAGISYS ALPMYLGELA PKNLRGMVGT MTEVFVIVGV181FLAQIFSLQA ILGNPAGWPV LLALTGVPAL LQLLTLPFFP ESPRYSLIQK GDEATARQAL241RRLRGHTDME AELEDMRAEA RAERAEGHLS VLHLCALRSL RWQLLSIIVL MAGQQLSGIN301AINYYADTIY TSAGVEAAHS QYVTVGSGVV NIVMTITSAV LVERLGRRHL LLAGYGICGS361ACLVLTVVLL FQNRVPELSY LGIICVFAYI AGHSIGPSPV PSVVRTEIFL QSSRRAAFMV421DGAVHWLTNF IIGFLFPSIQ EAIGAYSFII FAGICLLTAI YIYVVIPETK GKTFVEINRI481FAKRNRVKLP EEKEETIDAG PPTASPAKET SFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
512 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.