Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC2A5 / GLUT5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99945-100
Catalog Number: LS-G99945
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glucose Transporter 5 GLUT5(SLC2A5) (NM_003039), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Fructose transporter | Glucose transporter 5 | GLUT-5 | GLUT5 | SLC2A5
- NCBI GenBank
- NM_003039
- SwissProt
- P22732
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQQDQSMKE GRLTLVLALA TLIAAFGSSF QYGYNVAAVN SPALLMQQFY NETYYGRTGE61FMEDFPLTLL WSVTVSMFPF GGFIGSLLVG PLVNKFGRKG ALLFNNIFSI VPAILMGCSR121VATSFELIII SRLLVGICAG VSSNVVPMYL GELAPKNLRG ALGVVPQLFI TVGILVAQIF181GLRNLLANVD GWPILLGLTG VPAALQLLLL PFFPESPRYL LIQKKDEAAA KKALQTLRGW241DSVDREVAEI RQEDEAEKAA GFISVLKLFR MRSLRWQLLS IIVLMGGQQL SGVNAIYYYA301DQIYLSAGVP EEHVQYVTAG TGAVNVVMTF CAVFVVELLG RRLLLLLGFS ICLIACCVLT361AALALQDTVS WMPYISIVCV ISYVIGHALG PSPIPALLIT EIFLQSSRPS AFMVGGSVHW421LSNFTVGLIF PFIQEGLGPY SFIVFAVICL LTTIYIFLIV PETKAKTFIE INQIFTKMNK481VSEVYPEKEE LKELPPVTSE QTRTRPLEQK LISEEDLAAN DILDYKDDDD KV
532 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.