Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC2A4RG / GEF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111333-100
Catalog Number: LS-G111333
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC2A4RG (NM_020062), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GLUT4 enhancer factor | HDBP1 | SLC2A4 regulator | SLC2A4RG | Si-1-2 | Si-1-2-19 | GEF | HDBP-1
- Origin Species
- Human
- NCBI GenBank
- NM_020062
- SwissProt
- Q9NR83
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MERPPPRAAG RDPSALRAEA PWLRAEGPGP RAAPVTVPTP PQGSSVGGGF AGLEFARPQE61SEPRASDLGA PRTWTGAAAG PRTPSAHIPV PAQRATPGKA RLDEVMAAAA LTSLSTSPLL121LGAPVAAFSP EPGLEPWKEA LVRPPGSYSS SSNSGDWGWD LASDQSSPST PSPPLPPEAA181HFLFGEPTLR KRKSPAQVMF QCLWKSCGKV LSTASAMQRH IRLVHLGRQA EPEQSDGEED241FYYTELDVGV DTLTDGLSSL TPVSPTASMP PAFPRLELPE LLEPPALPSP LRPPAPPLPP301PPVLSTVANP QSCHSDRVYQ GCLTPARLEP QPTEVGACPP ALSSRIGVTL RKPRGDAKKC361RKVYGMERRD LWCTACRWKK ACQRFLDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
387 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.