Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC2A11 / GLUT11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121099-100
Catalog Number: LS-G121099
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC2A11 (NM_001024938), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Glucose transporter protein 10 | Glucose transporter type 10 | GLUT-10 | GLUT10 | Glucose transporter type 11 | GLUT-11 | GLUT11 | SLC2A11 | SLC2A11-a | SLC2A11-c | Glucose transporter protein 11
- Origin Species
- Human
- NCBI GenBank
- NM_030807
- SwissProt
- Q9BYW1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRALRRLIQG RILLLTICAA GIGGTFQFGY NLSIINAPTL HIQEFTNETW QARTGEPLPD61HLVLLMWSLI VSLYPLGGLF GALLAGPLAI TLGRKKSLLV NNIFVVSAAI LFGFSRKAGS121FEMIMLGRLL VGVNAGVSMN IQPMYLGESA PKELRGAVAM SSAIFTALGI VMGQVVGLRE181LLGGPQAWPL LLASCLVPGA LQLASLPLLP ESPRYLLIDC GDTEACLAAL RRLRGSGDLA241GELEELEEER AACQGCRARR PWELFQHRAL RRQVTSLVVL GSAMELCGND SVYAYASSVF301RKAGVPEAKI QYAIIGTGSC ELLTAVVSCV VIERVGRRVL LIGGYSLMTC WGSIFTVALC361LQSSFPWTLY LAMACIFAFI LSFGIGPAGV TGILATELFD QMARPAACMV CGALMWIMLI421LVGLGFPFIM EALSHFLYVP FLGVCVCGAI YTGLFLPETK GKTFQEISKE LHRLNFPRRA481QGPTWRSLEV IQSTELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.