Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC26A6 / PAT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102226-100
Catalog Number: LS-G102226
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC26A6 (NM_134263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Anion transporter 1 | Pendrin-L1 | Pendrin-like protein 1 | Sulfate anion transporter | CFEX | PAT1 | Pendrin L1 | SLC26A6
- Origin Species
- Human
- NCBI GenBank
- NM_022911
- SwissProt
- Q9BXS9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLADASGPR DTQALLSATQ AMDLRRRDYH MERPLLNQEH LEELGRWGSA PRTHQWRTWL61QCSRARAYAL LLQHLPVLVW LPRYPVRDWL LGDLLSGLSV AIMQLPQGLA YALLAGLPPV121FGLYSSFYPV FIYFLFGTSR HISVGTFAVM SVMVGSVTES LAPQALNDSM INETARDAAR181VQVASTLSVL VGLFQVGLGL IHFGFVVTYL SEPLVRGYTT AAAVQVFVSQ LKYVFGLHLS241SHSGPLSLIY TVLEVCWKLP QSKVGTVVTA AVAGVVLVVV KLLNDKLQQQ LPMPIPGELL301TLIGATGISY GMGLKHRFEV DVVGNIPAGL VPPVAPNTQL FSKLVGSAFT IAVVGFAIAI361SLGKIFALRH GYRVDSNQEL VALGLSNLIG GIFQCFPVSC SMSRSLVQES TGGNSQVAGA421ISSLFILLII VKLGELFHDL PKAVLAAIII VNLKGMLRQL SDMRSLWKAN RADLLIWLVT481FTATILLNLD LGLVVAVIFS LLLVVVRTQM PHYSVLGQVP DTDIYRDVAE YSEAKEVRGV541KVFRSSATVY FANAEFYSDA LKQRCGVDVD FLISQKKKLL KKQEQLKLKQ LQKEEKLRKQ601AASPKGASVS INVNTSLEDM RSNNVEDCKM MQVSSGDKME DATANGQEDS KAPDGSTLKA661LGLPQPDFHS LILDLGALSF VDTVCLKSLK NIFHDFREIE VEVYMAACHS PVVSQLEAGH721FFDASITKKH LFASVHDAVT FALQHPRPVP DSPVSVTRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
759 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.