Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC25A24 / APC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102672-100
Catalog Number: LS-G102672
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC25A24 (NM_213651), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APC1 | Phosphate carrier) | member 24 | SLC25A24 | Calcium-binding transporter | MCSC1 | SCAMC-1 | SCAMC1
- Origin Species
- Human
- NCBI GenBank
- NM_013386
- SwissProt
- Q6NUK1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSLYGDLFW YLDYNKDGTL DIFELQEGLE DVGAIQSLEE AKKIFTTGDV NKDGKLDFEE61FMKYLKDHEK KMKLAFKSLD KNNDGKIEAS EIVQSLQTLG LTISEQQAEL ILQSIDVDGT121MTVDWNEWRD YFLFNPVTDI EEIIRFWKHS TGIDIGDSLT IPDEFTEDEK KSGQWWRQLL181AGGIAGAVSR TSTAPLDRLK IMMQVHGSKS DKMNIFGGFR QMVKEGGIRS LWRGNGTNVI241KIAPETAVKF WAYEQYKKLL TEEGQKIGTF ERFISGSMAG ATAQTFIYPM EVMKTRLAVG301KTGQYSGIYD CAKKILKHEG LGAFYKGYVP NLLGIIPYAG IDLAVYELLK SYWLDNFAKD361SVNPGVMVLL GCGALSSTCG QLASYPLALV RTRMQAQAML EGSPQLNMVG LFRRIISKEG421IPGLYRGITP NFMKVLPAVG ISYVVYENMK QTLGVTQKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
458 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.