Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC24A2 / NCKX2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130593-100
Catalog Number: LS-G130593
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NCKX2(SLC24A2) (NM_001193288), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NCKX2 | Retinal cone Na-Ca+K exchanger | SLC24A2
- Origin Species
- Human
- NCBI GenBank
- NM_020344
- SwissProt
- Q9UI40
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLQQSTTIT SLEKWCLDES LSGCRRHYSV KKKLKLIRVL GLFMGLVAIS TVSFSISAFS61ETDTQSTGEA SVVSGPRVAQ GYHQRTLLDL NDKILDYTPQ PPLSKEGESE NSTDHAQGDY121PKDIFSLEER RKGAIILHVI GMIYMFIALA IVCDEFFVPS LTVITEKLGI SDDVAGATFM181AAGGSAPELF TSLIGVFIAH SNVGIGTIVG SAVFNILFVI GMCALFSREI LNLTWWPLFR241DVSFYIVDLI MLIIFFLDNV IMWWESLLLL TAYFCYVVFM KFNVQVEKWV KQMINRNKVV301KVTAPEAQAK PSAARDKDEP TLPAKPRLQR GGSSASLHNS LMRNSIFQLM IHTLDPLAEG361RFREKASILH KIAKKKCHVD ENERQNGAAN HVEKIELPNS TSTDVEMTPS SDASEPVQNG421NLSHNIEGAE AQTADEEEDQ PLSLAWPSET RKQVTFLIVF PIVFPLWITL PDVRKPSSRK481FFPITFFGSI TWIAVFSYLM VWWAHQVGET IGISEEIMGL TILAAGTSIP DLITSVIVAR541KGLGDMAVSS SVGSNIFDIT VGLPLPWLLY TVIHRFQPVA VSSNGLFCAI VLLFIMLLFV601ILSIALCKWR MNKILGFIMF GLYFVFLVVS VLLEDRILTC PVSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
644 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.