Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC23A2 / SVCT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116169-100
Catalog Number: LS-G116169
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC23A2 (NM_005116), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0238 | HSVCT2 | SLC23A2 | SVCT2 | NBTL1 | YSPL2 | Vitamin C transporter 2
- Origin Species
- Human
- NCBI GenBank
- NM_005116
- SwissProt
- Q9UGH3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMGIGKNTTS KSMEAGSSTE GKYEDEAKHP AFFTLPVVIN GGATSSGEQD NEDTELMAIY61TTENGIAEKS SLAETLDSTG SLDPQRSDMI YTIEDVPPWY LCIFLGLQHY LTCFSGTIAV121PFLLADAMCV GYDQWATSQL IGTIFFCVGI TTLLQTTFGC RLPLFQASAF AFLAPARAIL181SLDKWKCNTT DVSVANGTAE LLHTEHIWYP RIREIQGAII MSSLIEVVIG LLGLPGALLK241YIGPLTITPT VALIGLSGFQ AAGERAGKHW GIAMLTIFLV LLFSQYARNV KFPLPIYKSK301KGWTAYKLQL FKMFPIILAI LVSWLLCFIF TVTDVFPPDS TKYGFYARTD ARQGVLLVAP361WFKVPYPFQW GLPTVSAAGV IGMLSAVVAS IIESIGDYYA CARLSCAPPP PIHAINRGIF421VEGLSCVLDG IFGTGNGSTS SSPNIGVLGI TKVGSRRVIQ CGAALMLALG MIGKFSALFA481SLPDPVLGAL FCTLFGMITA VGLSNLQFID LNSSRNLFVL GFSIFFGLVL PSYLRQNPLV541TGITGIDQVL NVLLTTAMFV GGCVAFILDN TIPGTPEERG IRKWKKGVGK GNKSLDGMES601YNLPFGMNII KKYRCFSYLP ISPTFVGYTW KGLRKSDNSR SSDEDSQATGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
650 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.