Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC23A1 / SVCT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106088-100
Catalog Number: LS-G106088
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC23A1 (NM_152685), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MSVCT1 | HSVCT1 | SVCT1 | Vitamin C transporter 1 | YSPL3 | SLC23A1
- Origin Species
- Human
- NCBI GenBank
- AJ269477
- SwissProt
- Q9UHI7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRAQEDLEGR TQHETTRDPS TPLPTEPKFD MLYKIEDVPP WYLCILLGFQ HYLTCFSGTI61AVPFLLAEAL CVGHDQHMVS QLIGTIFTCV GITTLIQTTV GIRLPLFQAS AFAFLVPAKA121ILALERWKCP PEEEIYGNWS LPLNTSHIWH PRIREVGLHV QGAIMVSSVV EVVIGLLGLP181GALLNYIGPL TVTPTVSLIG LSVFQAAGDR AGSHWGISAC SILLIILFSQ YLRNLTFLLP241VYRWGKGLTL LRIQIFKMFP IMLAIMTVWL LCYVLTLTDV LPTDPKAYGF QARTDARGDI301MAIAPWIRIP YPCQWGLPTV TAAAVLGMFS ATLAGIIESI GDYYACARLA GAPPPPVHAI361NRGIFTEGIC CIIAGLLGTG NGSTSSSPNI GVLGITKVGS RRVVQYGAAI MLVLGTIGKF421TALFASLPDP ILGGMFCTLF GMITAVGLSN LQFVDMNSSR NLFVLGFSMF FGLTLPNYLE481SNPGAINTGI LEVDQILIVL LTTEMFVGGC LAFILDNTVP GSPEERGLIQ WKAGAHANSD541MSSSLKSYDF PIGMGIVKRI TFLKYIPICP VFKGFSSSSK DQIAIPEDTP ENTETASVCT601KVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
602 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.