Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC22A9 / OAT7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107724-100
Catalog Number: LS-G107724
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC22A9 (NM_080866), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HOAT4 | OAT4 | Organic anion transporter 4 | SLC22A9 | UST3H | OAT7 | Organic anion transporter 7 | HUST3 | Ust3
- Origin Species
- Human
- NCBI GenBank
- NM_080866
- SwissProt
- Q8IVM8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAFQDLLGHA GDLWRFQILQ TVFLSIFAVA TYLHFMLENF TAFIPGHRCW VHILDNDTVS61DNDTGALSQD ALLRISIPLD SNMRPEKCRR FVHPQWQLLH LNGTFPNTSD ADMEPCVDGW121VYDRISFSST IVTEWDLVCD SQSLTSVAKF VFMAGMMVGG ILGGHLSDRF GRRFVLRWCY181LQVAIVGTCA ALAPTFLIYC SLRFLSGIAA MSLITNTIML IAEWATHRFQ AMGITLGMCP241SGIAFMTLAG LAFAIRDWHI LQLVVSVPYF VIFLTSSWLL ESARWLIINN KPEEGLKELR301KAAHRSGMKN ARDTLTLEIL KSTMKKELEA AQKKKPSLCE MLHMPNICKR ISLLSFTRFA361NFMAYFGLNL HVQHLGNNVF LLQTLFGAVI LLANCVAPWA LKYMNRRASQ MLLMFLLAIC421LLAIIFVPQE MQTLREVLAT LGLGASALAN TLAFAHGNEV IPTIIRARAM GINATFANIA481GALAPLMMIL SVYSPPLPWI IYGVFPFISG FAFLLLPETR NKPLFDTIQD EKNERKDPRE541PKQEDPRVEV TQFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
553 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.