Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC22A8 / OAT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100249-100
Catalog Number: LS-G100249
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC22A8 (NM_004254), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HOAT3 | OAT3 | SLC22A8 | ROat3 | Roct | Organic anion transporter 3
- Origin Species
- Human
- NCBI GenBank
- NM_004254
- SwissProt
- Q8TCC7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTFSEILDRV GSMGHFQFLH VAILGLPILN MANHNLLQIF TAATPVHHCR PPHNASTGPW61VLPMGPNGKP ERCLRFVHPP NASLPNDTQR AMEPCLDGWV YNSTKDSIVT EWDLVCNSNK121LKEMAQSIFM AGILIGGLVL GDLSDRFGRR PILTCSYLLL AASGSGAAFS PTFPIYMVFR181FLCGFGISGI TLSTVILNVE WVPTRMRAIM STALGYCYTF GQFILPGLAY AIPQWRWLQL241TVSIPFFVFF LSSWWTPESI RWLVLSGKSS KALKILRRVA VFNGKKEEGE RLSLEELKLN301LQKEISLAKA KYTASDLFRI PMLRRMTFCL SLAWFATGFA YYSLAMGVEE FGVNLYILQI361IFGGVDVPAK FITILSLSYL GRHTTQAAAL LLAGGAILAL TFVPLDLQTV RTVLAVFGKG421CLSSSFSCLF LYTSELYPTV IRQTGMGVSN LWTRVGSMVS PLVKITGEVQ PFIPNIIYGI481TALLGGSAAL FLPETLNQPL PETIEDLENW SLRAKKPKQE PEVEKASQRI PLQPHGPGLG541SSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
542 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.