Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC1A7 / EAAT5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100879-100
Catalog Number: LS-G100879
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC1A7 (NM_006671), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AAAT | EAAT5 | Retinal glutamate transporter | SLC1A7
- Origin Species
- Human
- NCBI GenBank
- NM_006671
- SwissProt
- O00341
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF QFPGELLMRM61LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT FMAVIVGIFM VSIIHPGSAA121QKETTEQSGK PIMSSADALL DLIRNMFPAN LVEATFKQYR TKTTPVVKSP KVAPEEAPPR181RILIYGVQEE NGSHVQNFAL DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG241DSGAPLVSFC QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV301VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI TFKCLLENNH361IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL DFGQIITISI TATAASIGAA421GIPQAGLVTM VIVLTSVGLP TDDITLIIAV DWALDRFRTM INVLGDALAA GIMAHICRKD481FARDTGTEKL LPCETKPVSL QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVERDEE541LPAASLNHCT IQISELETNVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
560 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.