
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC16A7 / MCT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100376-100
Catalog Number: LS-G100376
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MCT2(SLC16A7) (NM_004731), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MCT 2 | Monocarboxylate transporter 2 | SLC16A7 | MCT2
- Origin Species
- Human
- NCBI GenBank
- NM_004731
- SwissProt
- O60669
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPMPSAPPV HPPPDGGWGW IVVGAAFISI GFSYAFPKAV TVFFKEIQQI FHTTYSEIAW61ISSIMLAVMY AGGPVSSVLV NKYGSRPVVI AGGLLCCLGM VLASFSSSVV QLYLTMGFIT121GLGLAFNLQP ALTIIGKYFY RKRPMANGLA MAGSPVFLSS LAPFNQYLFN TFGWKGSFLI181LGSLLLNACV AGSLMRPLGP NQTTSKSKNK TGKTEDDSSP KKIKTKKSTW EKVNKYLDFS241LFKHRGFLIY LSGNVIMFLG FFAPIIFLAP YAKDQGIDEY SAAFLLSVMA FVDMFARPSV301GLIANSKYIR PRIQYFFSFA IMFNGVCHLL CPLAQDYTSL VLYAVFFGLG FGSVSSVLFE361TLMDLVGAPR FSSAVGLVTI VECGPVLLGP PLAGKLVDLT GEYKYMYMSC GAIVVAASVW421LLIGNAINYR LLAKERKEEN ARQKTRESEP LSKSKHSEDV NVKVSNAQSV TSERETNITRTRPLEQKLISEEDLAANDILDYKDDDDKV
478 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
