Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC16A6 / MCT6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116483-100
Catalog Number: LS-G116483
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MCT7(SLC16A6) (NM_004694), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Breast cancer protein BCY5 | MCT 6 | MCT6 | Monocarboxylate transporter 7 | MCT 7 | MCT7 | Monocarboxylate transporter 6 | SLC16A6
- Origin Species
- Human
- NCBI GenBank
- NM_004694
- SwissProt
- O15403
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTQNKLKLCS KANVYTEVPD GGWGWAVAVS FFFVEVFTYG IIKTFGVFFN DLMDSFNESN61SRISWIISIC VFVLTFSAPL ATVLSNRFGH RLVVMLGGLL VSTGMVAASF SQEVSHMYVA121IGIISGLGYC FSFLPTVTIL SQYFGKRRSI VTAVASTGEC FAVFAFAPAI MALKERIGWR181YSLLFVGLLQ LNIVIFGALL RPIFIRGPAS PKIVIQENRK EAQYMLENEK TRTSIDSIDS241GVELTTSPKN VPTHTNLELE PKADMQQVLV KTSPRPSEKK APLLDFSILK EKSFICYALF301GLFATLGFFA PSLYIIPLGI SLGIDQDRAA FLLSTMAIAE VFGRIGAGFV LNREPIRKIY361IELICVILLT VSLFAFTFAT EFWGLMSCSI FFGFMVGTIG GTHIPLLAED DVVGIEKMSS421AAGVYIFIQS IAGLAGPPLA GLLVDQSKIY SRAFYSCAAG MALAAVCLAL VRPCKMGLCQ481HHHSGETKVV SHRGKTLQDI PEDFLEMDLA KNEHRVHVQM EPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
523 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.