Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC16A2 / MCT8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115257-100
Catalog Number: LS-G115257
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC16A2 (NM_006517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Allan-Herndon-Dudley syndrome | AHDS | DXS128E | DXS128 | MCT8 | Monocarboxylate transporter 7 | Monocarboxylate transporter 8 | MCT 7 | MCT 8 | MRX22 | XPCT | MCT7 | SLC16A2
- Origin Species
- Human
- NCBI GenBank
- NM_006517
- SwissProt
- P36021
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRGGGGLDV GGGGEGSRDR LSRDGLASWG AEPGGGGSGS GSSSPPSSSS CSSRNKYQPQ61SGSSGPSSHS PPAAMALQSQ ASEEAKGPWQ EADQEQQEPV GSPEPESEPE PEPEPEPVPV121PPPEPQPEPQ PLPDPAPLPE LEFESERVHE PEPTPTVETR GTARGFQPPE GGFGWVVVFA181ATWCNGSIFG IHNSVGILYS MLLEEEKEKN RQVEFQAAWV GALAMGMIFF CSPIVSIFTD241RLGCRITATA GAAVAFIGLH TSSFTSSLSL RYFTYGILFG CGCSFAFQPS LVILGHYFQR301RLGLANGVVS AGSSIFSMSF PFLIRMLGDK IKLAQTFQVL STFMFVLMLL SLTYRPLLPS361SQDTPSKRGV RTLHQRFLAQ LRKYFNMRVF RQRTYRIWAF GIAAAALGYF VPYVHLMKYV421EEEFSEIKET WVLLVCIGAT SGLGRLVSGH ISDSIPGLKK IYLQVLSFLL LGLMSMMIPL481CRDFGGLIVV CLFLGLCDGF FITIMAPIAF ELVGPMQASQ AIGYLLGMMA LPMIAGPPIA541GLLRNCFGDY HVAFYFAGVP PIIGAVILFF VPLMHQRMFK KEQRDSSKDK MLAPDPDPNG601ELLPGSPNPE EPITRTRPLEQKLISEEDLAANDILDYKDDDDKV
613 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.