Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC15A1 / PEPT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116220-100
Catalog Number: LS-G116220
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC15A1 (NM_005073), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPECT1 | PEPT1 | SLC15A1 | HPEPT1 | Peptide transporter 1
- Origin Species
- Human
- NCBI GenBank
- NM_005073
- SwissProt
- P46059
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGMSKSHSFF GYPLSIFFIV VNEFCERFSY YGMRAILILY FTNFISWDDN LSTAIYHTFV61ALCYLTPILG ALIADSWLGK FKTIVSLSIV YTIGQAVTSV SSINDLTDHN HDGTPDSLPV121HVVLSLIGLA LIALGTGGIK PCVSAFGGDQ FEEGQEKQRN RFFSIFYLAI NAGSLLSTII181TPMLRVQQCG IHSKQACYPL AFGVPAALMA VALIVFVLGS GMYKKFKPQG NIMGKVAKCI241GFAIKNRFRH RSKAFPKREH WLDWAKEKYD ERLISQIKMV TRVMFLYIPL PMFWALFDQQ301GSRWTLQATT MSGKIGALEI QPDQMQTVNA ILIVIMVPIF DAVLYPLIAK CGFNFTSLKK361MAVGMVLASM AFVVAAIVQV EIDKTLPVFP KGNEVQIKVL NIGNNTMNIS LPGEMVTLGP421MSQTNAFMTF DVNKLTRINI SSPGSPVTAV TDDFKQGQRH TLLVWAPNHY QVVKDGLNQK481PEKGENGIRF VNTFNELITI TMSGKVYANI SSYNASTYQF FPSGIKGFTI SSTEIPPQCQ541PNFNTFYLEF GSAYTYIVQR KNDSCPEVKV FEDISANTVN MALQIPQYFL LTCGEVVFSV601TGLEFSYSQA PSNMKSVLQA GWLLTVAVGN IIVLIVAGAG QFSKQWAEYI LFAALLLVVC661VIFAIMARFY TYINPAEIEA QFDEDEKKNR LEKSNPYFMS GANSQKQMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
708 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.