Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC14A1 / JK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124810-100
Catalog Number: LS-G124810
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC14A1 (NM_001128588), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Kidd Blood Group | HsT1341 | HUT11 | RACH1 | RACH2 | SLC14A1 | SLC14A1 JK | Urea transporter 1 | UT-B1 | Urea transporter-B1 | UT1 | UTE | JK | Urea transporter | erythrocyte | UT-B
- Origin Species
- Human
- NCBI GenBank
- NM_015865
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNGRSLIGGA GDARHGPVWK DPFGTKAGDA ARRGIARLSL ALADGSQEQE PEEEIAMEDS61PTMVRVDSPT MVRGENQVSP CQGRRCFPKA LGYVTGDMKE LANQLKDKPV VLQFIDWILR121GISQVVFVNN PVSGILILVG LLVQNPWWAL TGWLGTVVST LMALLLSQDR SLIASGLYGY181NATLVGVLMA VFSDKGDYFW WLLLPVCAMS MTCPIFSSAL NSMLSKWDLP VFTLPFNMAL241SMYLSATGHY NPFFPAKLVI PITTAPNISW SDLSALELLK SIPVGVGQIY GCDNPWTGGI301FLGAILLSSP LMCLHAAIGS LLGIAAGLSL SAPFENIYFG LWGFNSSLAC IAMGGMFMAL361TWQTHLLALG CALFTAYLGV GMANFMAEVG LPACTWPFCL ATLLFLIMTT KNSNIYKMPL421SKVTYPEENR IFYLQAKKRM VESPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.