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SLC13A5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SLC13A5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SLC13A5 antibodies →
$504.00each
Catalog Number: LS-G104825-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC13A5 (NM_177550), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Na(+)/citrate cotransporter | NACT | SLC13A5
Origin Species
Human
SwissProt
Q86YT5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASALSYVSK FKSFVILFVT PLLLLPLVIL MPAKFVRCAY VIILMAIYWC TEVIPLAVTS
61LMPVLLFPLF QILDSRQVCV QYMKDTNMLF LGGLIVAVAV ERWNLHKRIA LRTLLWVGAK
121PARLMLGFMG VTALLSMWIS NTATTAMMVP IVEAILQQME ATSAATEAGL ELVDKGKAKE
181LPGSQVIFEG PTLGQQEDQE RKRLCKAMTL CICYAASIGG TATLTGTGPN VVLLGQMNEL
241FPDSKDLVNF ASWFAFAFPN MLVMLLFAWL WLQFVYMRFN FKKSWGCGLE SKKNEKAALK
301VLQEEYRKLG PLSFAEINVL ICFFLLVILW FSRDPGFMPG WLTVAWVEGE TKYVSDATVA
361IFVATLLFIV PSQKPKFNFR SQTEEERKTP FYPPPLLDWK VTQEKVPWGI VLLLGGGFAL
421AKGSEASGLS VWMGKQMEPL HAVPPAAITL ILSLLVAVFT ECTSNVATTT LFLPIFASMS
481RSIGLNPLYI MLPCTLSASF AFMLPVATPP NAIVFTYGHL KVADMVKTGV IMNIIGVFCV
541FLAVNTWGRA IFDLDHFPDW ANVTHIET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

568 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.