Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC13A2 / NaCT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128292-100
Catalog Number: LS-G128292
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC13A2 (NM_001145975), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NACT | NaDC-1 | NADC1 | SLC13A2 | SDCT1 | HNaDC-1
- Origin Species
- Human
- NCBI GenBank
- NM_003984
- SwissProt
- Q13183
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATCWQALWA YRSYLIVFFV PILLLPLPIL VPSKEAYCAY AIILMALFWC TEALPLAVTA61LFPLILFPMM GIVDASEIIQ RPFPSSFESP GECQSVGMSV TASHNLGGTV GDSRVFPPLS121HVSTCQVAVE YLKDSNLLFF GGLLVAIAVE HWNLHKRIAL RVLLIVGVRP APLILGFMLV181TAFLSMWISN TATSAMMVPI AHAVLDQLHS SQASSNVEEG SNNPTFELQE PSPQKEVTKL241DNGQALPVTS ASSEGRAHLS QKHLHLTQCM SLCVCYSASI GGIATLTGTA PNLVLQGQIN301SLFPQNGNVV NFASWFSFAF PTMVILLLLA WLWLQILFLG FNFRKNFGIG EKMQEQQQAA361YCVIQTEHRL LGPMTFAEKA ISILFVILVL LWFTREPGFF LGWGNLAFPN AKGESMVSDG421TVAIFIGIIM FIIPSKFPGL TQDPENPGKL KAPLGLLDWK TVNQKMPWNI VLLLGGGYAL481AKGSERSGLS EWLGNKLTPL QSVPAPAIAI ILSLLVATFT ECTSNVATTT IFLPILASMA541QAICLHPLYV MLPCTLATSL AFMLPVATPP NAIVFSFGDL KVLDMARAGF LLNIIGVLII601ALAINSWGIP LFSLHSFPSW AQSNTTAQCL PSLANTTTPS PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
641 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.