Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC12A6 / KCC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119552-100
Catalog Number: LS-G119552
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLC12A6 (NM_001042495), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACCPN | KCC3A | KCC3 | SLC12A6 | K-Cl cotransporter 3 | KCC3B
- Origin Species
- Human
- NCBI GenBank
- NM_005135
- SwissProt
- Q9UHW9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 120.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEMSGATTS LATVALDPPS DRTSHPQDVI EDLSQNSITG EHSQLLDDGH KKARNAYLNN61SNYEEGDEYF DKNLALFEEE MDTRPKVSSL LNRMANYTNL TQGAKEHEEA ENITEGKKKP121TKTPQMGTFM GVYPPCLQNI FGVILFLRLT WVVGTAGVLQ AFAIVLICCC CTMLTAISMS181AIATNGVVPA GGSYFMISRA LGPEFGGAVG LCFYLGTTFA AAMYILGAIE IFLVYIVPRA241AIFHSDDALK ESAAMLNNMR VYGTAFLVLM VLVVFIGVRY VNKFASLFLA CVIVSILAIY301AGAIKSSFAP PHFPVCMLGN RTLSSRHIDV CSKTKEINNM TVPSKLWGFF CNSSQFFNAT361CDEYFVHNNV TSIQGIPGLA SGIITENLWS NYLPKGEIIE KPSAKSSDVL GSLNHEYVLV421DITTSFTLLV GIFFPSVTGI MAGSNRSGDL KDAQKSIPIG TILAILTTSF VYLSNVVLFG481ACIEGVVLRD KFGDAVKGNL VVGTLSWPSP WVIVIGSFFS TCGAGLQSLT GAPRLLQAIA541KDNIIPFLRV FGHSKANGEP TWALLLTAAI AELGILIASL DLVAPILSMF FLMCYLFVNL601ACALQTLLRT PNWRPRFRYY HWALSFMGMS ICLALMFISS WYYAIVAMVI AGMIYKYIEY661QGAEKEWGDG IRGLSLSAAR FALLRLEEGP PHTKNWRPQL LVLLKLDEDL HVKHPRLLTF721ASQLKAGKGL TIVGSVIVGN FLENYGEALA AEQTIKHLME AEKVKGFCQL VVAAKLREGI781SHLIQSCGLG GMKHNTVVMG WPNGWRQSED ARAWKTFIGT VRVTTAAHLA LLVAKNISFF841PSNVEQFSEG NIDVWWIVHD GGMLMLLPFL LKQHKVWRKC SIRIFTVAQL EDNSIQMKKD901LATFLYHLRI EAEVEVVEMH DSDISAYTYE RTLMMEQRSQ MLRHMRLSKT ERDREAQLVK961DRNSMLRLTS IGSDEDEETE TYQEKVHMTW TKDKYMASRG QKAKSMEGFQ DLLNMRPDQS1021NVRRMHTAVK LNEVIVNKSH EAKLVLLNMP GPPRNPEGDE NYMEFLEVLT EGLERVLLVR1081GGGSEVITIY STRTRPLEQKLISEEDLAANDILDYKDDDDKV
1091 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.