Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC12A5 / KCC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101684-100
Catalog Number: LS-G101684
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCC2(SLC12A5) (NM_020708), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Erythroid K-Cl cotransporter 2 | HKCC2 | K-Cl cotransporter 2 | KCC2 | KIAA1176 | SLC12A5 | Neuronal K-Cl cotransporter
- Origin Species
- Human
- NCBI GenBank
- NM_020708
- SwissProt
- Q9H2X9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLNNLTDCED GDGGANPGDG NPKESSPFIN STDTEKGKEY DGKNMALFEE EMDTSPMVSS61LLSGLANYTN LPQGSREHEE AENNEGGKKK PVQAPRMGTF MGVYLPCLQN IFGVILFLRL121TWVVGIAGIM ESFCMVFICC SCTMLTAISM SAIATNGVVP AGGSYYMISR SLGPEFGGAV181GLCFYLGTTF AGAMYILGTI EILLAYLFPA MAIFKAEDAS GEAAAMLNNM RVYGTCVLTC241MATVVFVGVK YVNKFALVFL GCVILSILAI YAGVIKSAFD PPNFPICLLG NRTLSRHGFD301VCAKLAWEGN ETVTTRLWGL FCSSRFLNAT CDEYFTRNNV TEIQGIPGAA SGLIKENLWS361SYLTKGVIVE RSGMTSVGLA DGTPIDMDHP YVFSDMTSYF TLLVGIYFPS VTGIMAGSNR421SGDLRDAQKS IPTGTILAIA TTSAVYISSV VLFGACIEGV VLRDKFGEAV NGNLVVGTLA481WPSPWVIVIG SFFSTCGAGL QSLTGAPRLL QAISRDGIVP FLQVFGHGKA NGEPTWALLL541TACICEIGIL IASLDEVAPI LSMFFLMCYM FVNLACAVQT LLRTPNWRPR FRYYHWTLSF601LGMSLCLALM FICSWYYALV AMLIAGLIYK YIEYRGAEKE WGDGIRGLSL SAARYALLRL661EEGPPHTKNW RPQLLVLVRV DQDQNVVHPQ LLSLTSQLKA GKGLTIVGSV LEGTFLENHP721QAQRAEESIR RLMEAEKVKG FCQVVISSNL RDGVSHLIQS GGLGGLQHNT VLVGWPRNWR781QKEDHQTWRN FIELVRETTA GHLALLVTKN VSMFPGNPER FSEGSIDVWW IVHDGGMLML841LPFLLRHHKV WRKCKMRIFT VAQMDDNSIQ MKKDLTTFLY HLRITAEVEV VEMHESDISA901YTYEKTLVME QRSQILKQMH LTKNEREREI QSITDESRGS IRRKNPANTR LRLNVPEETA961GDSEEKPEEE VQLIHDQSAP SCPSSSPSPG EEPEGEGETD PEKVHLTWTK DKSVAEKNKG1021PSPVSSEGIK DFFSMKPEWE NLNQSNVRRM HTAVRLNEVI VKKSRDAKLV LLNMPGPPRN1081RNGDENYMEF LEVLTEHLDR VMLVRGGGRE VITIYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1116 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.