Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SKAP / HSD11 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108306-100
Catalog Number: LS-G108306
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRAF4AF1(KNSTRN) (NM_033286), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C15orf23 | SKAP | TRAF4AF1 | HSD11 | KNSTRN
- Origin Species
- Human
- NCBI GenBank
- W17330
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPEAPPLD RVFRTTWLST ECDSHPLPPS YRKFLFETQE ADLAGGTTVA AGNLLNESEK61DCGQDRRAPG VQPCLLVTMT SVVKTVYSLQ PSSALSGGQP ADTQTRATSK SLLPVRSKEV121DVSKQLHSGG PENDVTKITK LRRENGQMKA TDTATRRNVR KGYKPLSKQK SEEELKDKNQ181LLEAVNKQLH QKLTETQGEL KDLTQKVELL EKFRDNCLAI LESKGLDPAL GGETLASRQE241STTDHMDSML LLETLQEELK LFNETAKKQM EELQALKVKL EMKEERVRFL EQQTLCNNQV301NDLTTALKEM EQLLEMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
316 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.