Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SIRT7 / Sirtuin 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101447-100
Catalog Number: LS-G101447
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIRT7 (NM_016538), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SIRT7 | SIR2L7 | Sirtuin type 7 | SIR2-like protein 7 | Sir2-related protein type 7 | Sirtuin 7
- Origin Species
- Human
- NCBI GenBank
- NM_016538
- SwissProt
- Q9NRC8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAGGLSRSE RKAAERVRRL REEQQRERLR QVSRILRKAA AERSAEEGRL LAESADLVTE61LQGRSRRREG LKRRQEEVCD DPEELRGKVR ELASAVRNAK YLVVYTGAGI STAASIPDYR121GPNGVWTLLQ KGRSVSAADL SEAEPTLTHM SITRLHEQKL VQHVVSQNCD GLHLRSGLPR181TAISELHGNM YIEVCTSCVP NREYVRVFDV TERTALHRHQ TGRTCHKCGT QLRDTIVHFG241ERGTLGQPLN WEAATEAASR ADTILCLGSS LKVLKKYPRL WCMTKPPSRR PKLYIVNLQW301TPKDDWAALK LHGKCDDVMR LLMAELGLEI PAYSRWQDPI FSLATPLRAG EEGSHSRKSL361CRSREEAPPG DRGAPLSSAP ILGGWFGRGC TKRTKRKKVTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
400 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.