LSBio
SIPA1L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIPA1L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110964-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIPA1L2 (NM_020808), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1389 | SIPA1L2 | SPAL2 | SPA-1-like 2 | SIPA1-like protein 2
Origin Species
Human
NCBI GenBank
AA166678

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
190.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDPRQSQEE KHKLGRASSK FKDPPRIMQS DDYFARKFKA INGNMGPTTS LNASNSNETG
61GGGPANGTPA VPKMGVRARV SEWPPKKDCS KELTCKALWE SRSQTSYESI TSVLQNGQSD
121QSEGQQDEQL DLDFVEAKYT IGDIFVHSPQ RGLHPIRQRS NSDVTISDID AEDVLDQNAV
181NPNTGAALHR EYGSTSSIDR QGLSGENFFA MLRGYRVENY DHKAMVPFGF PEFFRCDPAI
241SPSLHAAAQI SRGEFVRISG LDYVDSALLM GRDRDKPFKR RLKSESVETS LFRKLRTVKS
301EHETFKFTSE LEESRLERGI RPWNCQRCFA HYDVQSILFN INEAMATRAN VGKRKNITTG
361ASAASQTQMP TGQTGNCESP LGSKEDLNSK ENLDADEGDG KSNDLVLSCP YFRNETGGEG
421DRRIALSRAN SSSFSSGESC SFESSLSSHC TNAGVSVLEV PRENQPIHRE KVKRYIIEHI
481DLGAYYYRKF FYGKEHQNYF GIDENLGPVA VSIRREKVED AKEKEGSQFN YRVAFRTSEL
541TTLRGAILED AIPSTARHGT ARGLPLKEVL EYVIPELSIQ CLRQASNSPK VSEQLLKLDE
601QGLSFQHKIG ILYCKAGQST EEEMYNNETA GPAFEEFLDL LGQRVRLKGF SKYRAQLDNK
661TDSTGTHSLY TTYKDYELMF HVSTLLPYMP NNRQQLLRKR HIGNDIVTIV FQEPGALPFT
721PKSIRSHFQH VFVIVKVHNP CTENVCYSVG VSRSKDVPPF GPPIPKGVTF PKSAVFRDFL
781LAKVINAENA AHKSEKFRAM ATRTRQEYLK DLAENFVTTA TVDTSVKFSF ITLGAKKKEK
841VKPRKDAHLF SIGAIMWHVI ARDFGQSADI ECLLGISNEF IMLIEKDSKN VVFNCSCRDV
901IGWTSGLVSI KVFYERGECV LLSSVDNCAE DIREIVQRLV IVTRGCETVE MTLRRNGLGQ
961LGFHVNFEGI VADVEPFGFA WKAGLRQGSR LVEICKVAVA TLTHEQMIDL LRTSVTVKVV
1021IIQPHDDGSP RRGCSELCRI PMVEYKLDSE GTPCEYKTPF RRNTTWHRVP TPALQPLSRA
1081SPIPGTPDRL PCQQLLQQAQ AAIPRSTSFD RKLPDGTRSS PSNQSSSSDP GPGGSGPWRP
1141QVGYDGCQSP LLLEHQGSGP LECDGARERE DTMEASRHPE TKWHGPPSKV LGSYKERALQ
1201KDGSCKDSPN KLSHIGDKSC SSHSSSNTLS SNTSSNSDDK HFGSGDLMDP ELLGLTYIKG
1261ASTDSGIDTA PCMPATILGP VHLAGSRSLI HSRAEQWADA ADVSGPDDEP AKLYSVHGYA
1321STISAGSAAE GSMGDLSEIS SHSSGSHHSG SPSAHCSKSS GSLDSSKVYI VSHSSGQQVP
1381GSMSKPYHRQ GAVNKYVIGW KKSEGSPPPE EPEVTECPGM YSEMDVMSTA TQHQTVVGDA
1441VAETQHVLSK EDFLKLMLPD SPLVEEGRRK FSFYGNLSPR RSLYRTLSDE SICSNRRGSS
1501FGSSRSSVLD QALPNDILFS TTPPYHSTLP PRAHPAPSMG SLRNEFWFSD GSLSDKSKCA
1561DPGLMPLPDT ATGLDWTHLV DAARAFEGLD SDEELGLLCH HTSYLDQRVA SFCTLTDMQH
1621GQDLEGAQEL PLCVDPGSGK EFMDTTGERS PSPLTGKVNQ LELILRQLQT DLRKEKQDKA
1681VLQAEVQHLR QDNMRLQEES QTATAQLRKF TEWFFTTIDK KS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1722 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.