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SIMC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIMC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SIMC1 antibodies →
$504.00each
Catalog Number: LS-G103628-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5orf25(SIMC1) (NM_198567), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C5orf25 | OOMA1 | SIMC1
Origin Species
Human
NCBI GenBank
NM_198567

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPASASGED LRKLPTMAEV NGEQDFIDLT RETRPRTKDR SGLYVIDLTR AEGENRPIAT
61LDLTLEPVTP SQKEPTSLQT CASLSGKAVM EGHVDRSSQP TARRIINSDP VDLDLVEENT
121FVGPPPATSI SGGSVYPTEP NCSSATFTGN LSFLASLQLS SDVSSLSPTS NNSRSSSSSS
181NQKAPLPCPQ QDVSRPPQAL PCPLRPLPCP PRASPCPPRA SSCPPRALSC PSQTMQCQLP
241ALTHPPQEVP CPRQNIPGPP QDSLGLPQDV PGLPQSILHP QDVAYLQDMP RSPGDVPQSP
301SDVSPSPDAP QSPGGMPHLP GDVLHSPGDM PHSSGDVTHS PRDIPHLPGD RPDFTQNDVQ
361NRDMPMDISA LSSPSCSPRP QSETPLEKVP WLSVMETPAR KEISLSEPAK PGSAHVQSRT
421PQGGLYNRPC LHRLKYFLRP PVHHLFFQTL IPDKDTRENK GQRLEPIPHR RLRMVTNTIE
481ENFPLGTVQF LMDFVSPQHY PPREIVAHII QKILLSGSET VDVLKEAYML LMKIQQLHPA
541NAKTVEWDWK LLTYVMEEEG QTLPGRVLFL RYVVQTLEDD FQQTLRRQRQ HLQQSIANMV
601LSCDKQPHNV RDVIKWLVKA VTEDGLTQPP NGNQTSSGTG ILKASSSHPS SQPNLTKNTN
661QLIVCQLQRM LSIAVEVDRT PTCSSNKIAE MMFGFVLDIP ERSQREMFFT TMESHLLRCK
721VLEIIFLHSC ETPTRLPLSL AQALYFLNNS TSLLKCQSDK SQWQTWDELV ERLQFLLSSY
781QHVLREHLRS SVIDRKDLII KRIKPKPQQG DDITVVDVEK QIEAFRSRLI QMLGEPLVPQ
841LQDKVHLLKL LLFYAADLNP DAEPFQKGWS GS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

872 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.