Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SIK3 / QSK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109553-100
Catalog Number: LS-G109553
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIK3 (NM_025164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0999 | L19 | QSK | Salt-inducible kinase 3 | SIK-3 | SIK3 | SIK family kinase 3
- Origin Species
- Human
- NCBI GenBank
- NM_025164
- SwissProt
- Q9Y2K2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 139.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KTQLDEENLK KIFREVQIMK61MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG RMAEKEARRK FKQIVTAVYF121CHCRNIVHRD LKAENLLLDA NLNIKIADFG FSNLFTPGQL LKTWCGSPPY AAPELFEGKE181YDGPKVDIWS LGVVLYVLVC GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML241VLDPNKRLSM EQICKHKWMK LGDADPNFDR LIAECQQLKE ERQVDPLNED VLLAMEDMGL301DKEQTLQSLR SDAYDHYSAI YSLLCDRHKR HKTLRLGALP SMPRALAFQA PVNIQAEQAG361TAMNISVPQV QLINPENQIV EPDGTLNLDS DEGEEPSPEA LVRYLSMRRH TVGVADPRTE421VMEDLQKLLP GFPGVNPQAP FLQVAPNVNF MHNLLPMQNL QPTGQLEYKE QSLLQPPTLQ481LLNGMGPLGR RASDGGANIQ LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ541EAVQSSTYKD SNTLHLPTER FSPVRRFSDG AASIQAFKAH LEKMGNNSSI KQLQQECEQL601QKMYGGQIDE RTLEKTQQQH MLYQQEQHHQ ILQQQIQDSI CPPQPSPPLQ AACENQPALL661THQLQRLRIQ PSSPPPNHPN NHLFRQPSNS PPPMSSAMIQ PHGAASSSQF QGLPSRSAIF721QQQPENCSSP PNVALTCLGM QQPAQSQQVT IQVQEPVDML SNMPGTAAGS SGRGISISPS781AGQMQMQHRT NLMATLSYGH RPLSKQLSAD SAEAHSLNVN RFSPANYDQA HLHPHLFSDQ841SRGSPSSYSP STGVGFSPTQ ALKVPPLDQF PTFPPSAHQQ PPHYTTSALQ QALLSPTPPD901YTRHQQVPHI LQGLLSPRHS LTGHSDIRLP PTEFAQLIKR QQQQRQQQQQ QQQQQEYQEL961FRHMNQGDAG SLAPSLGGQS MTERQALSYQ NADSYHHHTS PQHLLQIRAQ ECVSQASSPT1021PPHGYAHQPA LMHSESMEED CSCEGAKDGF QDSKSSSTLT KGCHDSPLLL STGGPGDPES1081LLGTVSHAQE LGIHPYGHQP TAAFSKNKVP SREPVIGNCM DRSSPGQAVE LPDHNGLGYP1141ARPSVHEHHR PRALQRHHTI QNSDDAYVQL DNLPGMSLVA GKALSSARMS DAVLSQSSLM1201GSQQFQDGEN EECGASLGGH EHPDLSDGSQ HLNSSCYPST CITDILLSYK HPEVSFSMEQ1261AGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1263 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.