Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SIGLEC5 / CD170 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117059-100
Catalog Number: LS-G117059
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIGLEC5 (NM_003830), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD170 antigen | CD170 | CD33 antigen-like 2 | OB-binding protein 2 | SIGLEC-5 | SIGLEC5 | CD33L2 | OB-BP2 | OBBP2 | Obesity-binding protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_003830
- SwissProt
- O15389
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPLLLLPLL WGGSLQEKPV YELQVQKSVT VQEGLCVLVP CSFSYPWRSW YSSPPLYVYW61FRDGEIPYYA EVVATNNPDR RVKPETQGRF RLLGDVQKKN CSLSIGDARM EDTGSYFFRV121ERGRDVKYSY QQNKLNLEVT ALIEKPDIHF LEPLESGRPT RLSCSLPGSC EAGPPLTFSW181TGNALSPLDP ETTRSSELTL TPRPEDHGTN LTCQMKRQGA QVTTERTVQL NVSYAPQTIT241IFRNGIALEI LQNTSYLPVL EGQALRLLCD APSNPPAHLS WFQGSPALNA TPISNTGILE301LRRVRSAEEG GFTCRAQHPL GFLQIFLNLS VYSLPQLLGP SCSWEAEGLH CRCSFRARPA361PSLCWRLEEK PLEGNSSQGS FKVNSSSAGP WANSSLILHG GLSSDLKVSC KAWNIYGSQS421GSVLLLQGRS NLGTGVVPAA LGGAGVMALL CICLCLIFFL IVKARRKQAA GRPEKMDDED481PIMGTITSGS RKKPWPDSAG DQASPPGDAP PLEEQKELHY ASLSFSEMKS REPKDQEAPS541TTEYSEIKTS KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
551 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.