LSBio
SIGLEC12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIGLEC12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SIGLEC12 antibodies →
$504.00each
Catalog Number: LS-G108057-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIGLECL1(SIGLEC12) (NM_053003), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Siglec-XII | SLG | Siglec-12 | S2V | Siglec-L1 | SIGLEC12 | SIGLEC-like 1
Origin Species
Human
SwissProt
Q96PQ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLLLLLLPP LLCGRVGAKE QKDYLLTMQK SVTVQEGLCV SVLCSFSYPQ NGWTASDPVH
61GYWFRAGDHV SRNIPVATNN PARAVQEETR DRFHLLGDPQ NKDCTLSIRD TRESDAGTYV
121FCVERGNMKW NYKYDQLSVN VTASQDLLSR YRLEVPESVT VQEGLCVSVP CSVLYPHYNW
181TASSPVYGSW FKEGADIPWD IPVATNTPSG KVQEDTHGRF LLLGDPQTNN CSLSIRDARK
241GDSGKYYFQV ERGSRKWNYI YDKLSVHVTA LTHMPTFSIP GTLESGHPRN LTCSVPWACE
301QGTPPTITWM GASVSSLDPT ITRSSMLSLI PQPQDHGTSL TCQVTLPGAG VTMTRAVRLN
361ISYPPQNLTM TVFQGDGTAS TTLRNGSALS VLEGQSLHLV CAVDSNPPAR LSWTWGSLTL
421SPSQSSNLGV LELPRVHVKD EGEFTCRAQN PLGSQHISLS LSLQNEYTGK MRPISGVTLG
481AFGGAGATAL VFLYFCIIFV VVRSCRKKSA RPAVGVGDTG MEDANAVRGS ASQGPLIESP
541ADDSPPHHAP PALATPSPEE GEIQYASLSF HKARPQYPQE QEAIGYEYSE INIPK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

595 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.